Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Antibodies

Article Snippet: MT-CO2 , Rabbit , Cell Signaling Technology , #31,219.

Techniques:

Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Didymin improves mitochondrial function in PBCs. ( A ) MitoTracker Green staining for mitochondrial content in INS-1 cells. Scale bar = 30 μm. (n = 4) ( B ) Western blot analysis of NRF1, TFAM, NDUFB8, SDHB and MTCO2 in INS-1 cells (n = 3). ( C ) Mitochondrial oxygen consumption ratio (OCR) of INS-1 cells (n = 4). ( D ) ROS concentration in INS-1 cells (n = 4). ( E ) Analysis of mitochondrial structure of PBCs in mice by electron microscopy. Scale bar = 0.6 μm. ( F ) Immunofluorescence staining of COX-IV (green) and insulin (red) in pancreas. Scale bar = 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001 PA vs. PA + Didymin. # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 control vs. PA.

Article Snippet: MT-CO2 , Rabbit , Cell Signaling Technology , #31,219.

Techniques: Staining, Western Blot, Concentration Assay, Electron Microscopy, Immunofluorescence, Control

Journal: bioRxiv

Article Title: The human mitochondrial 12S rRNA m 4 C methyltransferase METTL15 is required for proper mitochondrial function

doi: 10.1101/809756

Figure Lengend Snippet: (A) Comparison of mitochondrial genome (mtDNA) copy number between the Control and METTL15 KO cells, measured by RT-qPCR. (B) Expression analysis by RT-qPCR of mitochondrial coding genes in the control and METTL15 KO cells. (C) The protein level of COX2 is downregulated in successfully edited cell lines (red fonts) but not changed in parental or unedited cells (black fonts). (D) Western blot analysis of the indicated proteins in the control, METTL15 KO, and METTL15 KO cells rescued with the wildtype or catalytic mutant of METTL15-Flag-HA, respectively. LMNB1 and Actin were used as controls.

Article Snippet: Anti-COX2 (55070-1-AP)antibody was purchased from Proteintech.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Mutagenesis

Journal: bioRxiv

Article Title: The human mitochondrial 12S rRNA m 4 C methyltransferase METTL15 is required for proper mitochondrial function

doi: 10.1101/809756

Figure Lengend Snippet: (A) The distribution of the mitochondrial ribosome small and large subunits in the indicated sucrose gradient fractions, examined by 12S and 16S rRNA RT-qPCR. (B) The distribution of the mRNAs of the mitochondrial coding genes in the indicated sucrose gradient fractions examined by RT-qPCR. (C) Western blot analyses of ND6 and COX2 protein levels in the control and METTL15 KO cells. The bar graph represents the quantification results of 2 replicate experiments.

Article Snippet: Anti-COX2 (55070-1-AP)antibody was purchased from Proteintech.

Techniques: Quantitative RT-PCR, Western Blot

Journal: iScience

Article Title: LETMD1 regulates mitochondrial protein synthesis and import to guard brown fat mitochondrial integrity and function

doi: 10.1016/j.isci.2024.110944

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti-MTCO2 , Proteintech , Cat #: 55070-1-AP, RRID: AB_10859832.

Techniques: Recombinant, Modification, Saline, Reverse Transcription, Bicinchoninic Acid Protein Assay, Western Blot, Software

Journal: Endocrine-Related Cancer

Article Title: Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

doi: 10.1530/erc-15-0540

Figure Lengend Snippet: Figure 7. Western blotting analysis of cyclooxygenase-2 (COX2), phosphorylated signal transducer and activator of transcription 3 (pSTAT3), factor nuclear kappa B (NFkB), insulin-like growth factor receptor 1 (IGFR1) protein levels in the ventral prostate of the experimental groups. Different lowercase letters indicate

Article Snippet: The COX2, STAT3, 159 IGFR1, antigens were detected, respectively, using the following antibodies: mouse 160 monoclonal anti COX2 (sc-376861- Santa Cruz Biotechnology, Santa Cruz, CA, United 161 States of America), rabbit polyclonal anti-STAT3 (sc-7179 - Santa Cruz Biotechnology, 162 Santa Cruz, CA, USA), rabbit polyclonal anti- IGFR1 (sc-712- Santa Cruz Biotechnology, 163 Santa Cruz, CA, USA), mouse monoclonal anti-PCNA (ab-29 – Abcam, Cambridge, MA, 164 USA).

Techniques: Western Blot

Journal: Endocrine-Related Cancer

Article Title: Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

doi: 10.1530/erc-15-0540

Figure Lengend Snippet: Figure 8. COX2, STAT3, IGFR1 immunoreactivities in the ventral prostate of TRAMP mice from T8 (A, E, I), T12 (B, F, J) T1GTN (C, G, K) and T1CEL (D, H, L). Epithelial and stromal reactivities were graded according

Article Snippet: The COX2, STAT3, 159 IGFR1, antigens were detected, respectively, using the following antibodies: mouse 160 monoclonal anti COX2 (sc-376861- Santa Cruz Biotechnology, Santa Cruz, CA, United 161 States of America), rabbit polyclonal anti-STAT3 (sc-7179 - Santa Cruz Biotechnology, 162 Santa Cruz, CA, USA), rabbit polyclonal anti- IGFR1 (sc-712- Santa Cruz Biotechnology, 163 Santa Cruz, CA, USA), mouse monoclonal anti-PCNA (ab-29 – Abcam, Cambridge, MA, 164 USA).

Techniques:

Journal: Endocrine-Related Cancer

Article Title: Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

doi: 10.1530/erc-15-0540

Figure Lengend Snippet: Figure 9. COX2, STAT3, IGFR1 immunoreactivities in the ventral prostate of TRAMP mice from T22 (A, D, G,), T2GTN (B, E, H) and T2CEL (C, F, I). Epithelial and stromal reactivities were graded according to Table

Article Snippet: The COX2, STAT3, 159 IGFR1, antigens were detected, respectively, using the following antibodies: mouse 160 monoclonal anti COX2 (sc-376861- Santa Cruz Biotechnology, Santa Cruz, CA, United 161 States of America), rabbit polyclonal anti-STAT3 (sc-7179 - Santa Cruz Biotechnology, 162 Santa Cruz, CA, USA), rabbit polyclonal anti- IGFR1 (sc-712- Santa Cruz Biotechnology, 163 Santa Cruz, CA, USA), mouse monoclonal anti-PCNA (ab-29 – Abcam, Cambridge, MA, 164 USA).

Techniques: